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Optimal Antibody Purification Strategies Using Data-Driven Models Article
Songsong Liu, Lazaros G. Papageorgiou
Engineering 2019, Volume 5, Issue 6, Pages 1077-1092 doi: 10.1016/j.eng.2019.10.011
This work addresses the multiscale optimization of the purification processes of antibody fragments. Chromatography decisions in the manufacturing processes are optimized, including the number of chromatography columns and their sizes, the number of cycles per batch, and the operational flow velocities. Data-driven models of chromatography throughput are developed considering loaded mass, flow velocity, and column bed height as the inputs, using manufacturing-scale simulated datasets based on microscale experimental data. The piecewise linear regression modeling method is adapted due to its simplicity and better prediction accuracy in comparison with other methods. Two alternative mixed-integer nonlinear programming (MINLP) models are proposed to minimize the total cost of goods per gram of the antibody purification process, incorporating the data-driven models. These MINLP models are then reformulated as mixed-integer linear programming (MILP) models using linearization techniques and multiparametric disaggregation. Two industrially relevant cases with different chromatography column size alternatives are investigated to demonstrate the applicability of the proposed models.
Keywords: Antibody purification Multiscale optimization Antigen-binding fragment Mixed-integer programming
Frontiers of Medicine 2022, Volume 16, Issue 3, Pages 378-388 doi: 10.1007/s11684-021-0840-y
Keywords: severe Mycoplasma pneumoniae pneumonia children proteomics Fc fragment of the IgG-binding protein
Jiangnan Liu,Bin Yi,Zhe Zhang,Yi Cao
Frontiers of Medicine 2016, Volume 10, Issue 2, Pages 204-211 doi: 10.1007/s11684-016-0443-1
CD176 (Thomsen-Friedenreich antigen) is a tumor-associated carbohydrate epitope (glycotope) functionallyWe applied B lymphocytes obtained from mice immunized with CD176 antigen and constructed a phage displayA positive clone of CD176 single-chain variable antibody fragment (scFv) was successfully screened from
Keywords: CD176 Thomsen-Friedenreich antigen scFv cancer therapy adhesion metastasis
Chloride binding and time-dependent surface chloride content models for fly ash concrete
S. MUTHULINGAM,B. N. RAO
Frontiers of Structural and Civil Engineering 2016, Volume 10, Issue 1, Pages 112-120 doi: 10.1007/s11709-015-0322-x
Keywords: binding isotherms chloride ingress concrete fly ash surface chloride content
Chimeric antigen receptor T cell therapies for acute myeloid leukemia
Bin Gu, Jianhong Chu, Depei Wu
Frontiers of Medicine 2020, Volume 14, Issue 6, Pages 701-710 doi: 10.1007/s11684-020-0763-z
Keywords: acute myeloid leukemia CAR T immunotherapy
Xiaodong Mo, Xiaojun Huang
Frontiers of Medicine 2013, Volume 7, Issue 3, Pages 306-315 doi: 10.1007/s11684-013-0279-x
Keywords: partially matched related donor hematopoietic stem cell transplantation allogeneic
reduces IgE binding ability of allergenic egg white proteins
Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU
Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3, Pages 373-381 doi: 10.15302/J-FASE-2018210
Keywords: Aspergillus oryzae egg allergy egg white proteins IgE-binding ability ovomucoid
Yuan ZHANG,Yan ZHANG,Tao YU
Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 5, Pages 666-674 doi: 10.1007/s11783-013-0608-y
Keywords: binding ability dissolved organic matters fluorescence quenching complex capacity Taihu Lake
Repression of CDKN2C caused by PML/RARα binding promotes the proliferation and differentiation block
Xiaoling Wang,Yun Tan,Yizhen Li,Jingming Li,Wen Jin,Kankan Wang
Frontiers of Medicine 2016, Volume 10, Issue 4, Pages 420-429 doi: 10.1007/s11684-016-0478-3
Keywords: CDKN2C acute promyelocytic leukemia cell cycle arrest differentiation
A giant step forward: chimeric antigen receptor T-cell therapy for lymphoma
Houli Zhao, Yiyun Wang, Elaine Tan Su Yin, Kui Zhao, Yongxian Hu, He Huang
Frontiers of Medicine 2020, Volume 14, Issue 6, Pages 711-725 doi: 10.1007/s11684-020-0808-3
Keywords: chimeric antigen receptor T (CAR-T) cell lymphoma cytokine release syndrome (CRS) immune effector cell-associated
Serum carbohydrate antigen (CA) 19-9 as a prognostic factor in cholangiocarcinoma: A meta-analysis
Shang-Long LIU, Zi-Fang SONG, Qing-Gang HU, Shao-Bo HU, Jun LI, Qi-Chang ZHENG, Duo SHAN,
Frontiers of Medicine 2010, Volume 4, Issue 4, Pages 457-462 doi: 10.1007/s11684-010-0240-1
Keywords: cholangiocarcinoma prognostic factors serum carbohydrate antigen 19-9
CAR T cells redirected against tumor-specific antigen glycoforms: can low-sugar antigens guarantee a
Frontiers of Medicine 2022, Volume 16, Issue 3, Pages 322-338 doi: 10.1007/s11684-021-0901-2
Keywords: cancer immunotherapy chimeric antigen receptor solid tumors tumor-associated antigen glycosylation
Detection and monitoring prostate specific antigen using nanotechnology approaches to biosensing
Grant Perry, Fernando Cortezon-Tamarit, Sofia I. Pascu
Frontiers of Chemical Science and Engineering 2020, Volume 14, Issue 1, Pages 4-18 doi: 10.1007/s11705-019-1846-8
Keywords: biosensing immunosensor PSA prostate cancer fluorescence
Lili Zhou, Ping Li, Shiguang Ye, Xiaochen Tang, Junbang Wang, Jie Liu, Aibin Liang
Frontiers of Medicine 2020, Volume 14, Issue 6, Pages 786-791 doi: 10.1007/s11684-020-0751-3
Keywords: anti-CD19 chimeric antigen receptor T cell soft tissue bone marrow relapsed or refractory non-Hodgkin
Du Yan, Han Xue, Pu Rui, Xie Jiaxin, Zhang Yuwei, Cao Guangwen
Frontiers of Medicine 2014, Volume 8, Issue 2, Pages 217-226 doi: 10.1007/s11684-014-0326-2
This study was designed to investigate the contribution of miRNA-122-binding site polymorphism atA total of 1021 healthy controls, 302 HBV surface antigen (HBsAg) seroclearance subjects, and 2011 HBsAg-positiveAdditionally, rs3783553 was not significantly associated with chronic HBV infection, liver cirrhosis, HBV e antigen
Keywords: hepatocellular carcinoma (HCC) interaction miRNA-122-binding site IL-1A rs3783553 hepatitis B virus (
Title Author Date Type Operation
Optimal Antibody Purification Strategies Using Data-Driven Models
Songsong Liu, Lazaros G. Papageorgiou
Journal Article
Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein
Journal Article
CD176 single-chain variable antibody fragment inhibits the adhesion of cancer cells to endothelial cells
Jiangnan Liu,Bin Yi,Zhe Zhang,Yi Cao
Journal Article
Chloride binding and time-dependent surface chloride content models for fly ash concrete
S. MUTHULINGAM,B. N. RAO
Journal Article
Chimeric antigen receptor T cell therapies for acute myeloid leukemia
Bin Gu, Jianhong Chu, Depei Wu
Journal Article
Advancement of human leukocyte antigen-partially matched related hematopoietic stem cell transplantation
Xiaodong Mo, Xiaojun Huang
Journal Article
reduces IgE binding ability of allergenic egg white proteins
Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU
Journal Article
Quantitative characterization of Cu binding potential of dissolved organic matter (DOM) in sediment from
Yuan ZHANG,Yan ZHANG,Tao YU
Journal Article
Repression of CDKN2C caused by PML/RARα binding promotes the proliferation and differentiation block
Xiaoling Wang,Yun Tan,Yizhen Li,Jingming Li,Wen Jin,Kankan Wang
Journal Article
A giant step forward: chimeric antigen receptor T-cell therapy for lymphoma
Houli Zhao, Yiyun Wang, Elaine Tan Su Yin, Kui Zhao, Yongxian Hu, He Huang
Journal Article
Serum carbohydrate antigen (CA) 19-9 as a prognostic factor in cholangiocarcinoma: A meta-analysis
Shang-Long LIU, Zi-Fang SONG, Qing-Gang HU, Shao-Bo HU, Jun LI, Qi-Chang ZHENG, Duo SHAN,
Journal Article
CAR T cells redirected against tumor-specific antigen glycoforms: can low-sugar antigens guarantee a
Journal Article
Detection and monitoring prostate specific antigen using nanotechnology approaches to biosensing
Grant Perry, Fernando Cortezon-Tamarit, Sofia I. Pascu
Journal Article
may affect the survival of patients with relapsed or refractory non-Hodgkin lymphoma after chimeric antigen
Lili Zhou, Ping Li, Shiguang Ye, Xiaochen Tang, Junbang Wang, Jie Liu, Aibin Liang
Journal Article